Biogenic production of silver, zinc oxide, and cuprous oxide nanoparticles, and their impregnation into textiles with antiviral activity against SARS-CoV-2

Nanotechnology is being used to fight off infections caused by viruses, and one of the most outstanding nanotechnological uses is the design of protective barriers made of textiles functionalized with antimicrobial agents, with the challenge of combating the SARS-CoV-2 virus, the causal agent of COVID-19. This research is framed within two fundamental aspects: the first one is linked to the proposal of new methods of biogenic synthesis of silver, cuprous oxide, and zinc oxide nanoparticles using organic extracts as reducing agents. The second one is the application of nanomaterials in the impregnation (functionalization) of textiles based on methods called "in situ" (within the synthesis), and "post-synthesis" (after the synthesis), with subsequent evaluation of their effectiveness in reducing the viral load of SARS-CoV-2. The results show that stable, monodisperse nanoparticles with defined geometry can be obtained. Likewise, the "in situ" impregnation method emerges as the best way to adhere nanoparticles. The results of viral load reduction show that 'in situ' textiles with Cu2O NP achieved a 99.79% load reduction of the SARS-CoV-2 virus.

www.nature.com/scientificreports/ globulus (previously filtered); then, it was added drop by drop to the precursor solution maintaining the same temperature and agitation for 5 min. Next, sodium hydroxide ACS (NaOH) (CAS no. 1310-73-2) was added until the solution reached a pH of 10, maintaining stirring for 20 min. Once the procedure was completed, the textile sample was removed and left to drain for 10 min. Finally, the sample was placed in a forced convection oven (100 °C) for 15 min. For the 'post-synthesis' impregnation of Ag NP in textile, the same biogenic synthesis protocol described above was developed (without immersing the textile sample in the middle of the process). In this sense, 500 mL of Ag NP colloid was heated up to 60 °C and, then, the textile piece was immersed and kept for 20 min with magnetic stirring (400 rpm). Next, the sample was removed, washed with acetone and distilled water, and left to drain for 10 min. Finally, it was placed in a forced convection oven (100 °C) for 15 min.

Biogenic synthesis of cuprous oxide nanoparticles (Cu 2 O NP) and impregnation in textiles by
in situ and post-synthesis methods. The Cu 2 O NP synthesis was mediated by the green route. For this purpose, the juice of Myrciaria dubia "camu camu" was used as a reducing agent. In this case, the copper sulfate pentahydrate precursor (CuS0 4 5H 2 O) was used, preparing 450 mL at 0.05 M. The mixture was kept under magnetic stirring (400 rpm) for 10 min at room temperature. Next, the textile piece (70% cotton/30% polyester) was immersed and kept under stirring for 3 min. Then, 22.5 mL of 7.5 M sodium hydroxide (NaOH) was added. At this stage, the stirring was increased to 1200 rpm. The reduction process occurred when 90 mL of M. dubia juice was added dropwise under the same temperature and agitation conditions. The sample was kept for 10 min. Finally, it was removed, drained for 10 min, and placed in a forced convection oven (100 °C) for 15 min. This method corresponds to "in situ" textile impregnation. www.nature.com/scientificreports/ For "in situ" textile functionalization, Cu 2 O NP was synthesized using the same protocol described above but without considering adding the textile during the process. In this case, 500 mL of colloid was used, in which the textile piece was immersed and kept for 60 min with magnetic stirring (1200 rpm) at room temperature. Finally, the sample was removed, washed with acetone and distilled water, and allowed to drain for 10 min before being placed in a forced convection oven at 100 °C for 15 min.
Biogenic synthesis of zinc oxide nanoparticles (ZnO NP) and impregnation in textiles by "post-synthesis" method. The starting point for the synthesis of ZnO NP was the zinc acetate ACS precursor (CAS No. 5970- . For this, 30 mL of the precursor at 0.21 M was prepared. The reaction was carried out on a hotplate until a temperature of 70 °C was reached using magnetic stirring at 450 rpm. Next, 20 mL of aqueous extract of Coriandrum sativum was added with magnetic stirring (600 rpm) for 4 h. Finally, the sample was calcined in a muffle for 2 h at 500 °C.
The textile treatment was only for the "post-synthesis" process because the ZnO NP must go through a calcination process, which makes it impossible to do the treatment during the formation of nanostructures. In this case, 0.21 g of ZnO NP powder was diluted in 350 mL of ultrapure water and homogenized in ultrasound for 30 min at room temperature. Subsequently, the colloid was poured into a beaker and heated to 70 °C. The textile sample was then completely immersed and kept under magnetic stirring (400 rpm) for 1 h. Next, the sample was washed with acetone and distilled water, and left to drain for 10 min. Finally, the textile was placed in an oven at 100 °C for 15 min.
Textile treatment with Ag/Cu 2 O nanoparticles by "post-synthesis" method. The silver nanoparticles (Ag NP) and cuprous oxide nanoparticles (Cu 2 O NP) were synthesized separately based on the previously described method. The nanoparticles were impregnated by the "post-synthesis" method due to the nature of the final state of the colloids. For this purpose, the textile sample was immersed in 500 mL of Ag NP and kept in constant stirring (600 rpm) for 60 min at room temperature. Then, it was removed and left to drain for 10 min. Finally, the sample was dried in a forced convection oven (100 °C) for 15 min. The sample obtained, and previously impregnated with Ag NP, went through the impregnation with Cu 2 O NP, immersing the textile sample in 500 mL of Cu 2 O NP colloid for 60 min under constant stirring (1200 rpm), to finally remove it, let it drip-dry for 10 min and wash it with acetone and distilled water. Finally, the textile sample was dried in a forced convection oven at 100 °C for 15 min.
Characterization of nanoparticles. The NPs were initially characterized by UV-vis spectrophotometry (UV 1900, Shimadzu) in the range of 200-900 nm. The size and shape of the NPs were analyzed through transmission electron microscopy (TEM) by adding 5 µl of the colloid placed on a carbon-coated copper grid. Then, the NP sample was dried in a desiccator with silica for 16 h. Measurements were carried out on a JEOL (model JEM 2011) powered with a voltage acceleration of 120 kV. EDX mediations were carried out with the TEM equipped with an OXFORD EDS 6498. The crystal structure was analyzed by X-ray diffraction with a Cu Kα source in the 2θ range of 20-80°. FTIR analysis was performed to determine the main functional groups present in the NPs due to the presence of traces of the extracts. FTIR spectrum was collected at 4 cm −1 resolution and in the transmission mode (4000-500 cm −1 ) using an FTIR spectrophotometer (Nicolet 6700, Thermo Scientific). The spectra were analyzed with OMNIC 8.1 software (OMNIC Series 8.1.10, Thermo Fischer Scientific).
Obtainment of synthetic RNA from the E gene of the SARS-CoV-2 virus. Twenty positive samples with a Cq between 15 and 20 were selected. Subsequently, RNA was extracted using the GeneAid viral RNA Mini kit. The RNA obtained was quantified with digital equipment (NanoPhotometer ® NP80) and stored at − 80 °C.
RNA was treated with DNAase I, Thermo Fisher (#EN0521), and retrotranscribed using 10 µM oligodT, following the recommendation of Thermo Scientific RevertAid RT Kit (#K1691). Subsequently, PCR was performed to obtain the amplicons with the T7 polymerase binding site, using primers TIVF3 (TAA TAC GAC TCA CTA TAG GGA CCG ACG ACG ACG ACG ACT ACT AGC) and B3 (AGA GTA AAC GTA AAA AGA AGA AGG TT) which were migrated on 1.8% agarose gel. The amplicons were purified with Beckman Coulter AMPure XP microbeads-REF A63880, following the manufacturer's recommendations. RNA synthesis of the E-gene target region was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit for in vitro transcription from the purified amplicons. The in-vitro transcribed RNA was treated with DNAase I and purified using the Monarch® RNA Cleanup Kit T2040L, according to the manufacturer's recommendations. Finally, the RNA was quantified with a Qubit fluorometer.
Inoculum preparation. Twenty positive samples with Cq between 14-30 were selected from oro-nasopharyngeal swabs preserved in UTM (universal transport medium). 500 µL of each sample were mixed to obtain a 20 mL pool; a 30-s vortexing and spin-down were performed; 100 µL of this pool was aliquoted to evaluate the Cq; and the number of copies was identified. RNA was extracted using the GenElute™ Universal Total RNA Purification Kit, following the manufacturer's recommendation.
RT-qPCR standard curve for inoculum assessment. The standard curve was performed from RNA synthesized in vitro, using serial dilutions (1/10) with three replicates for each dilution. The number of copies was determined considering the molecular weight of the ssRNA sequence of the target region. In addition, the inoculum was assessed, considering 5 µL of extracted RNA. For RT-qPCR, the CAPITAL™ qRT-PCR Probe www.nature.com/scientificreports/ Mix kit was used, 4 × , 0.4 µM of primers E-Sarbeco-F1 and E-Sarbeco-R2, 0.2 µM of E-Sarbeco-P1 Probe, 0. 8 X of qPCR Probe Mix and 1 µL of Rtase with RNAse inhibitor, applying 50 °C reverse transcription for 10 min, followed by 95 °C denaturation for 3 min and 45 cycles of 95 °C for 10 s and 58 °C for 30 s 40 . The amplification process was performed on the mic qPCR thermal cycler, using probe hydrolysis as the chemistry type, selecting the FAM fluorochrome and the BHQ-1 Quencher, and activating the green channel in the elongation step.
To read the data, the threshold value was set, and the NTC (non-template control), CE (extraction control), and CP (positive control) controls were evaluated as comparison standards for the study samples. Finally, the equipment was configured to obtain the standard curve, and the Cq value of the inoculum was read and extrapolated to determine the number of copies.
Inoculation of functionalized textiles with SARS-CoV-2. 150 µL of SARS-CoV-2 inoculum was applied to two fabrics (70% cotton and 30% polyester) with the In Situ functionalization process with Ag NP (Ag-2,411 ppm) and Cu 2 O NP (Cu 2 O-734, 132 ppm), and four fabrics with Post Synthesis process with Ag NP (Ag-2,411 ppm), Cu 2 O NP (Cu 2 O-734,132 ppm), silver/copper (Ag/Cu 2 O 2.41 Ag ppm/734.13 Cu ppm) and ZnO NP (ZnO 398.7 ppm). Only the fabric with 70% cotton and 30% polyester without NP was used as a control. Three replicates of each NP-treated fabric were made.
The inoculated fabric was incubated at 18 °C for 20 h. Then, the inoculated area was swabbed; the swab end was placed in 1.5 mL tubes, and RNA was extracted with the GenElute™ Universal Total RNA Purification Kit, following the manufacturer's recommendation. RT-qPCR was performed as explained above. The viral readout was performed considering nanogram/µL concentration, which was then translated to the number of copies. The percentage of viral reduction was determined according to the equation: where B = the viral load of the inoculum, and A = the viral load of the NP treatment of the fabric.
Statistical analysis. The data represent the mean of three independent experiments and the standard deviation. Statistical comparisons were performed by one-way analysis of variance (ANOVA). All p-values less than 0.05 were considered statistically significant.

Results
Biogenic synthesis of silver nanoparticles (Ag NP). Figure 2a shows the X-ray diffraction (XRD) patterns for the colloidal Ag NP. Two peaks can be observed at the 38.1°and 43.5° positions corresponding to the (111) and (200) planes of silver as indicated by the JCPDS card, No. 04-0783. The results confirm that the Ag NPs are cubic crystals centered on the faces by the intensity of the (111) peak 41,42 . www.nature.com/scientificreports/ The morphology and size of the synthesized Ag NP were analyzed by TEM. The images shown in Fig. 2b indicate that the NPs have mostly spherical shapes, and only a minority have various polygonal and elongated shapes with an average size of 6 ± 3.7 nm. The NPs appear scattered with small agglomerations of various sizes 43,44 . EDS analysis shown in Fig. 2c confirms the formation of Ag NP.
Another technique used to confirm the formation and stability of the synthesized AgNPs was UV-vis spectrophotometry. Figure 2d shows the UV-vis absorption spectrum where a characteristic band for this material is observed, with its peak located at 412.5 nm. One of the authors of this research 45 developed a synthesis method for this type of nanomaterial via chemical route, obtaining a plasmon peak at 434 nm. Applying the Mie theory, the nanoparticle size was determined to be 59.2 nm. In contrast, this research shows a plasmon resonance peak at 412.5 nm, indicating a shift towards shorter wavelengths, suggesting a decrease in the effective size of the silver nanoparticles. This is corroborated by the average size result mentioned above. The results suggest a good reduction process and formation of nanostructure obtained by the biogenic route method.
FTIR spectra were used to identify the biomolecules possibly responsible for reducing silver ions for NPs formation in the synthesis process. The FTIR spectra show various functional groups that are related to organic molecules. Figure 2e shows this spectrum for a sample of Ag NP in a colloidal state and bio-reduced with eucalyptus extract. The broad and long peak located at 3411 cm −1 and the peak at 2916 cm −1 correspond to the hydroxyl (-OH) bonds by the vibration of the alcohol components and to the aldehyde C-H group by the vibration of the alkane components. The peak at 1591 cm -1 could correspond to the vibrations of the C-N bonds of the carboxyl group (-C=O). The peaks located at 1386 cm −1 and 1077 cm −1 correspond to the interactions of N-H amide and -C-N amine bonds because of possible silver nitrate waste. The lowest peak located at 780 cm −1 corresponds to small vibrations of alcohol bonds (O-H) associated with carboxyls and hydroxyls, which could participate in the synthesis process of NPs 46 . Figure 3 shows a broad spectrum of characterizations of Cu 2 O NP. X-ray diffraction patterns are shown in Fig. 3a, and the results show high crystallinity with noticeable diffraction angles of 23, 25, 28, 32, 34, 43, and 47° corresponding for Cu to a face-centered cubic structure 47,48 .

Biogenic synthesis of cuprous oxide nanoparticles (Cu 2 O NP).
TEM analysis (Fig. 3c) gives us information on the shape, size, and distribution of the NPs. It was determined that the NPs have irregular shapes and, in some cases, near-spherical shapes, with the formation of agglomerations. The average size of the synthesized Cu 2 O NP is 10 ± 2.92 nm, which is small compared to other publications 49 and similar to others synthesized by the green route too 50 . It is suggested that the size achieved is due to the influence of the plugging effect of the extract.
EDS analysis provides a quantitative analysis of the elements present in the formation of Cu 2 O NP. Figure 3b shows the presence of Cu but also a significant presence of oxygen, which suggests the formation of oxides in the synthesis process. The other elements are associated with traces derived from the initial reagents and the M. dubia extract. www.nature.com/scientificreports/ The formation of Cu 2 O NP was also confirmed by UV-vis spectrophotometry analysis (Fig. 3d). The absorption intensity was reached at 506.8 nm, characteristic of Cu 2 O NPs 48,51 . The broadening of the peak indicates polydisperse NPs.
The FTIR analysis is shown in Fig. 3e. The peak found at 3250 cm −1 belongs to the -OH groups due to the adsorption of water molecules, although it can also be related to the carboxyl group. At 1580 cm −1 there is a peak associated with the -C-N amine group, while the peak at 1380 cm −1 represents the presence of alkanes, and the peak at 1080 cm −1 indicates the -C-O group. The peak at 880 cm −1 represents the absorption of Cu-O-H bonds, indicating that functional groups play an important role in the synthesis of Cu 2 O NP, specifically in the reduction process. These results are similar to those reported by previous works 50,52 . Figure 4 shows the characterization results of the biogenic synthesis of ZnO NP. Figure 4a evidences the diffraction patterns of ZnO NP. All the diffraction peaks correspond to the characteristic hexagonal wurtzite structure and agree with the JCPDS card, No 36-1451 53,54 . The characteristic peaks correspond to the lattice planes (100), (002), (101), (102), (110), (103), and (112), with no peaks due to impurities observed. Figure 4b shows the EDS analysis confirming the formation of ZnO NP. The UV-vis absorption spectra of the ZnO NPs are shown in Fig. 4c, where the typical absorption peak that corresponds to this material is observed at 355.5 nm. However, a slight blue shift is observed in comparison to that found by other authors [55][56][57] . Figure 4d shows irregular hexagonal and near-spherical shapes with an average size of 50 ± 8.3 nm clustered in scattered agglomerations, similar to those found by Al-Kordy et al. 58 .

Textiles functionalized with Ag, Cu 2 O, ZnO and Ag/Cu 2 O nanomaterials.
The NPs were impregnated into the textile according to the previously described method. Figure 5 shows the SEM images of the textile fiber, which can be differentiated from the control (Fig. 5a) (without incorporation of NPs). In the case of Ag NP incorporation, as mentioned in the colloid section, quite small nanostructures (6 nm) have been achieved. The "in situ" impregnation method has generated that the NPs are formed in higher percentages in the fiber core and on the surface. Figure 5b represents the Ag NP treatment within situ impregnation, where small agglomerates can be observed. On the contrary, Fig. 5 c shows accumulations of higher quantities because the textile was immersed after having synthesized the Ag NP, finding agglomerations that finally cannot fully penetrate the fiber core, remaining in greater concentration on the surface. In the case of Cu 2 O NP (Fig. 5d), the images show that "in situ" impregnation has over-accumulated the textile fiber with nanomaterial through the presence of multiple layers, especially considering that the NPs had a concentration of 734.13 ppm, i.e., there is accumulation both in the core and on the surface. In the case of "post-synthesis" impregnation ( Fig. 5e), it can be noted that there is a non-saturated coating.
The ZnO NPs were obtained as a white powder, which was subsequently dissolved in ultrapure water for the textile immersion process. However, it was possible to observe in Fig. 5f the presence of small agglomerations   (Fig. 5g), which, being the same method, shows accumulations in large proportions around the fiber. The application of nanoparticles in the production of textiles has proven to be an effective strategy to improve their properties. However, one of the main challenges in incorporating nanoparticles into textiles is their stability within the textile matrix. In this regard, the stability of nanoparticles in impregnated textiles has been evaluated through various characterization techniques, such as SEM, EDS, and FTIR.
At a morphological level, colloid nanoparticles incorporated into the textile matrix have not undergone any changes. Moreover, energy-dispersive X-ray spectroscopy (EDS) ( Figure S1) shows the presence of elements, consolidating proper adherence and no change or reaction due to the presence of polyester material in the textile, ensuring elemental stability.
On the other hand, Fourier transform infrared spectroscopy (FTIR) ( Figure S1) is a technique that allows the identification of chemical interactions between nanoparticles and the textile matrix. This technique is based on measuring the absorption of infrared radiation by the sample and is used to determine the chemical characteristics of samples. The results of this technique show changes compared to the control textile, where the intensity of peaks, and even some peaks disappear when functionalized, which is possibly due to the appropriate nanoparticle density. Additionally, nanoparticles not only impregnate the surface but also penetrate the core of the textile fiber, allowing for stronger peaks of the nanomaterial to be shown compared to the corresponding groups in the control. Regarding the type of impregnation, changes can also be observed, where samples impregnated by the "Post-Synthesis" method show a lower intensity, which may be associated with a possible loss of nanomaterial on the surface.
The interactions between silver nanoparticles and cotton and polyester textiles can be electrostatic and chemical, favored by the positive charge of silver nanoparticles and the functional groups on the textile surface. In addition, silver nanoparticles can also adsorb onto the surface of the textile due to Van der Waals forces. These interactions can have an impact on the stability of nanoparticles in the textile and their ability to prevent the proliferation of microorganisms.
For the case of cuprous oxide nanoparticles, they have been obtained under alkaline conditions, and therefore the mentioned nanomaterial would have a negative charge due to the presence of cuprate ions on its surface, which in turn favors electrostatic interactions with positively charged groups on the textile surface, such as amino groups in the polyester fiber. In addition, functional groups on the surface of cuprous oxide nanoparticles can chemically interact with chemical groups in the textile. FTIR results of the nanomaterial show the presence of hydroxyl groups on the surface of cuprous oxide nanoparticles, which can interact with carboxyl groups on the surface of the textile, establishing strong chemical bonds.
In zinc oxide, the mechanism is similar, however, the importance of nanomaterial size is worth highlighting, as mentioned in the nanomaterial synthesis section. Better morphology and smaller nanostructures have been obtained with biogenic synthesis methods, so smaller nanoparticles can penetrate more deeply into textile fibers and have a greater surface area, increasing the likelihood of chemical bond formation with functional groups in the textile fibers.

Assessment of textiles functionalized against the SARS-CoV-2 virus.
The method of NP impregnation in the textile fiber plays an important role in the viricidal effects practiced in this research, linked to a better impregnation (in the core and surface of the fiber) which is translated into a higher concentration of nanomaterial. Thus, the "in situ" method shows better SARS-CoV-2 virus elimination results. Firstly, it is possible to compare the cases of textiles impregnated individually with Ag and Cu 2 O NP (Tables 1 and 2), where it is evident On the other hand, in an analysis according to the type of nanomaterial (Table 3), it can be evidenced the potential power of SARS-CoV-2 viral load reduction by Cu 2 O NP (99.79%) and ZnO NP (97.09%), values that are above the control (95.61%). However, the textile functionalized with Ag/Cu 2 O hybrid NPs shows a notorious reduction concerning the control (90.79%), most likely linked to an oxidation-reduction process between both types of NPs, which have reacted, leaving without effect potential properties at the level of electrical charges, which play an important role in the reduction of the viral load.

Discussion
As observed in the in-situ treatment, the viral load reduction values were > 95.56% between the treatments with silver or copper nanoparticles and the fabric control (a fabric used to impregnate the nanoparticles). It was demonstrated that there was a significant difference between the treatments, with the highest value of viral load reduction for the treatment with Cu 2 O NP (99.79%) being statistically significant in comparison with the other treatments (p < 0.05). A previous study using Cu 2 O NP against the Herpes virus showed a value of 83.3% (100 μg/mL) inhibition 59 . Cu 2 O may interfere with viral replication, the creation of ROS (Reactive oxygen species) radicals damaging the viral genome, and the denaturation of viral capsid proteins [59][60][61] . In the case of the antiviral effect on SARS-CoV-2 on copper-coated surfaces, it has been reported that the virus was not viable after 4 h post-exposure 36 . In addition, a study mentioned that the light-mediated plasmon effect on Cu 2 O NP enhances the antiviral effect 62 , so the use of clothing fabrics or face masks with NPs exposed to sunlight would enhance their antiviral effect. Another study using copper nanofibers in masks against SARS-CoV-2 showed 55% inhibition of viral replication after 48 h of exposure 63 , in contrast to our study with copper treatment that showed a 99.79% viral load decrease effect. Another previous study also showed that copper had good biocompatibility in toxicity and inflammatory response assays, so it is considered a safe metal for use 63 . In the case of Ag NP treatment, the decrease in viral load of SARS-CoV-2 was 95.56%, although with a lower significant effect compared to the control. A study using silver nitrate (AgNO 3 ) and silver oxide I (Ag 2 O) showed a potent antiviral effect against influenza A virus and that its effect depended on the solubility of the ion in water, presenting a higher antiviral effect when there is a higher ion solubility 61 . A previous study showed that the use of Ag NP (10 ppm) significantly decreased the number of viral copies (> 10 9 ) compared to the control 64 . In contrast, in our study, the Ag nanoparticle treatment decreased viral load by 95.56% in situ and 88% post-synthesis at 2.41 ppm. www.nature.com/scientificreports/ In Post Synthesis treatment, the highest antiviral effect resulted from using ZnO with a percentage of viral load reduction of 97.09% with a significant difference (p < 0.05) compared to the other treatments. A previous study has shown the antimicrobial effect of ZnO 65 . Another review study indicated that in, In vitro studies, the antiviral effect against coronavirus was through the inhibition of RNA polymerase 66 . Additionally, another study on fabrics impregnated with Zinc showed an inhibitory effect against SARS-CoV-2 and the influenza A virus 67 .
Other research using Zn salts demonstrated in In vitro studies an inactivation effect between 63 and 100% of the Herpes virus in a Zn concentration-dependent manner 68 . It has been determined that ZnO can generate free radicals capable of viral damage, inhibit protease and cause damage to viral RNA polymerase 69,70 . A recent study using ZnO NP against SARS-CoV-2 showed a 50% reduction in cytotoxicity caused by the virus in cell culture 71 . Additionally, Zn NP is an ideal antimicrobial and antiviral agent due to its low cytotoxicity 72 .
It was determined that the best treatment for in situ viral load reduction was copper, with 99.79% viral reduction, compared to the best treatment for post-synthesis viral load reduction, which was ZnO, with 97.09%. Therefore, the textile functionalized in situ with CuO NP was the treatment that showed the best effect on the viral load reduction, being statistically significant concerning the control and the other treatments.

Conclusions
The biogenic synthesis method presents a potential alternative for obtaining nanostructures with defined morphology, high stability, and monodispersity, highlighting the fundamental role of bioactive compounds that act as reducers and stabilizers of precursor metal salts for the formation of silver nanoparticles with diameters of 6 ± 3.7 nm, cuprous oxide nanoparticles of 10 ± 2.92 nm, and zinc oxide nanoparticles of 50 ± 8.3 nm. Furthermore, the obtained nanomaterial was applied in textile impregnation using techniques called 'in situ' and 'postsynthesis' , with 'in situ' treatment achieving better nanoparticle impregnation as evidenced by the formation of nanostructures in the core and surface of the textile fiber, while the 'post-synthesis' method shows agglomerations on the surface. On the other hand, it was determined that the type of nanomaterial and textile impregnation are associated with the reduction of viral load (SARS-CoV-2). Textiles impregnated with Cu 2 O nanoparticles showed the best reduction effect on SARS-CoV-2 viral load at 99.79%, followed by textiles functionalized with ZnO nanoparticles (97.09%), which, despite being the 'post-synthesis' method, exhibited good viricidal properties. Therefore, this technology can be employed in various areas of the healthcare sector and for inhibition of bacterial and fungal growth, as previous studies have highlighted the potential effects of these types of nanoparticles.

Data availability
The original data are available upon request from the corresponding author: davidasmat88@hotmail.com.